Documentation updates
- In previous versions, the functionality of the uniq_pepparameter (seecreate_dfhelp page) was incorrectly
described. We corrected the description to match its functionality as
follows: “Numerical. Proteins that are identified by this number or
fewer number of unique peptides are filtered out (default is 2).Only
applies wheninput_type = "MaxQuant".
Bug fixes
- In previous versions, volcano_plotfunction plotted
“-log10(P-value)” on the y-axis by default even whensig = "adjP"and only colored the dots by their
significance based on “adjP”. In this version,sig = "adjP"shows “-log10.adj. P-value” on the y-axis and colors the dots
by their “adjP” significance.
New data types allowed
- A new argument (data_type) added to thecreate_dffunction to accommodate other types of LFQ data
(raw intensity, iBAQ).
- A new argument input_typeadded to thecreate_dffunction to allow users to input data from a
standard quantitative matrix.
Workflow changes
- To allow for missing data imputation prior to or after data
normalization step (depending on the imputation method used), the
following changes were made:
- norm_dfand- imp_dfarguments replaced with
a generic- dfargument in the functions,- find_dep,- impute_na,- normalize_data, and- heatmap_de
- A note was added to the tutorials to clarify that for some
imputation methods, such as the kNN method, data normalization should be
performed prior to imputation.
 
Default machine learning
algorithms
- naive_bayesadded to the default- algorithm_listargument in the- train_modelsfunction.
Bug fixes
- Fixes a minor issue with create_dfwhen removing
potential contaminants. The number of potential contaminants removed is
now shown in the console.
- Fixes an issue with find_depthat previously used a
fixed value for theadj_methodargument.
- Fixes an issue with the file_pathargument for saving
the “TopHits.txt” file produced by thefind_depfunction.
Other changes
- Citation file updated with the biorxiv preprint details.
- Readme file updated with information on the Shiny App.
- Help pages updated.