NanoString nCounter data are gene expression assays where there is no need for the use of enzymes or amplification protocols and work with fluorescent barcodes (Geiss et al. (2018) <doi:10.1038/nbt1385>). Each barcode is assigned a messenger-RNA/micro-RNA (mRNA/miRNA) which after bonding with its target can be counted. As a result each count of a specific barcode represents the presence of its target mRNA/miRNA. 'NACHO' (NAnoString quality Control dasHbOard) is able to analyse the exported NanoString nCounter data and facilitates the user in performing a quality control. 'NACHO' does this by visualising quality control metrics, expression of control genes, principal components and sample specific size factors in an interactive web application.
| Version: | 2.0.6 | 
| Depends: | R (≥ 3.6.0) | 
| Imports: | utils, data.table, ggplot2 (≥ 3.3.0), ggforce (≥ 0.3.1), ggrepel (≥ 0.8.1), knitr (≥ 1.25), rmarkdown (≥ 1.16), shiny (≥ 1.4.0), shinyWidgets (≥ 0.4.9) | 
| Suggests: | roxygen2 (≥ 7.2.0), testthat (≥ 2.2.1), covr (≥ 3.3.2), Biobase, GEOquery, limma | 
| Published: | 2024-01-12 | 
| DOI: | 10.32614/CRAN.package.NACHO | 
| Author: | Mickaël Canouil | 
| Maintainer: | Mickaël Canouil <pro at mickael.canouil.dev> | 
| BugReports: | https://github.com/mcanouil/NACHO/issues | 
| License: | GPL-3 | 
| URL: | https://github.com/mcanouil/NACHO/, https://m.canouil.dev/NACHO/ | 
| NeedsCompilation: | no | 
| SystemRequirements: | pandoc (>= 1.14), pandoc-citeproc | 
| Citation: | NACHO citation info | 
| Materials: | README, NEWS | 
| In views: | Omics | 
| CRAN checks: | NACHO results | 
| Reference manual: | NACHO.html , NACHO.pdf | 
| Vignettes: | NACHO-analysis (source, R code) NACHO (source, R code) | 
| Package source: | NACHO_2.0.6.tar.gz | 
| Windows binaries: | r-devel: NACHO_2.0.6.zip, r-release: NACHO_2.0.6.zip, r-oldrel: NACHO_2.0.6.zip | 
| macOS binaries: | r-release (arm64): NACHO_2.0.6.tgz, r-oldrel (arm64): NACHO_2.0.6.tgz, r-release (x86_64): NACHO_2.0.6.tgz, r-oldrel (x86_64): NACHO_2.0.6.tgz | 
| Old sources: | NACHO archive | 
Please use the canonical form https://CRAN.R-project.org/package=NACHO to link to this page.